The HLA-B27 test is primarily ordered to help strengthen or confirm a suspected diagnosis of ankylosing spondylitis (AS), reactive arthritis, juvenile rheumatoid arthritis (JRA), or sometimes anterior uveitis.
ABO blood typing and HLA (tissue antigen) typing before a transplant helps ensure a close match. You will likely need to take medicine to suppress your immune system for the rest of your life to prevent the tissue from being rejected. Being careful about taking your post-transplant medicines and being closely watched by your doctor may help prevent rejection.
The goal of treatment is to make sure the transplanted organ or tissue works properly, and to suppress your immune system response. Suppressing the immune response may prevent transplant rejection.
Medicines will likely be used to suppress the immune response. Dosage depends on your condition and may be very high while the tissue is being rejected. After you no longer have signs of rejection, the dosage will likely be lowered.
Test Method 1 : Virus isolation and HSV-antibody testingSpecimens removed from virus-transport medium (veal infusion broth with antibiotics) were inoculated onto microtiter plates containing human diploid fibroblasts, and all isolates were typed by monoclonal antibodies, as described elsewhere . The same virus-isolation method was used throughout the study period. The HSV type_specific serological tests were done by Western blot
PCR assayThe PCR assay was a modification of the real-time quantitative fluorescent_probe assay described elsewhere . The swab samples in PCR buffer were thawed and opened, the swab was discarded, and 200 _L of the swab sample was extracted by use of Qiagen columns, as described elsewhere. Ten microliters of the extracted DNA was used in each assay. The primers and probes were directed to the HSV glycoprotein B gene